F4/80 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS80218-50, BS80218-100

Swiss-Prot: Q61549

Host: Rabbit

F4/80 Polyclonal Antibody-BS80218

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 65-250KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of variousLysates, using F4/80 antibody at 1:770 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of RAW264.7 cells using F4/80 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: DD7A5-7, EGF-TM7, Emr1, F4/80, Gpf480, Ly71, TM7LN3

Immunogen: Recombinant fusion protein of mouse F4/80(NP_034260.1).

Modification: Unmodified

Conjugate: Unconjugated